Janet Maylie has performed Off-Broadway in the original production of A.R. Gurney's The Dining Room at Playwrights Horizons and The Astor Place Theatre, in Fourtune at the Actors' Playhouse and in Approaching Zero at LaMaMa ETC. Off-Off Broadway, she appeared as Viola in Twelfth Night at the Alvina Krause Theatre, Susan B. Anthony in Chamber Music at the Walden Theatre and Doreen in The Private Ear.

Professor Maylie's regional theatre experience includes The Merry Wives of Windsor (Mrs. Ford) at the Meadow Brook Theatre, When You Comin' Back Red Ryder (Clarisse) at the New Mexico Repertory, Taking Steps (Elizabeth) at the Arizona Theatre Company, Bus Stop (Cherie) at The Barter Theatre, The Seven Keys to Baldpate (Mary), The Portable Pioneer and Prairie Show (Karen) at The American Stage Festival, The Ride Down Mt. Morgan (Theodora) at Performance Network, Off the Map (Arlene), Shoe Man (Sally), The Purple Rose Spring Comedy Festival, Completing Dahlia (Rachel), Blithe Spirit (Ruth) at the Purple Rose Theatre Company, and The Vast Difference (Dr. Howard) at the Purple Rose and The Gem Theatre. She has also been seen at the Festival of New Works in Rock Garden, Jack's Wife and The Immersion of Madeleine Favorini.

Her television appearances include Hill Street Blues, The Love Boat, As The World Turns, over 90 national and regional commercials, and numerous radio and television voiceovers. She has received four Theatre Excellence Awards from The Detroit Free Press, and is a member of SAG, AFTRA, and AEA.